The murine cyclooxygenase-2 complexed with a nido-dicarbaborate-containing indomethacin derivative

nido-Dicarbaborate Induces Potent and Selective Inhibition of Cyclooxygenase-2.

Cyclooxygenase-2,COX-2,Glucocorticoid-regulated inflammatory cyclooxygenase,Gripghs,Macrophage activation-associated marker protein P71/73,PES-2,PHS II,Prostaglandin H2 synthase 2,PGHS-2,Prostaglandin-endoperoxide synthase 2,TIS10 protein

mCOX-2 protein reconstituted with a 2-fold molar excess of heme in phosphtate buffer, pH 6.7, 100 mM NaCl, 1.2% (w/v) -OG, and 0.1% NaN3, and 10-fold molar excess of inhibitors from 25 mM DMSO stocks were added to protein samples. Mixing 3 uL of the protein-inhibitor complex with 3 uL crystallization solution containing 50 mM EPPS, pH 8.0, 120 mM MgCl2, 22-26% PEG MME-550 against reservoir solutions comprised of 50 mM EPPS pH 8.0, 120 mM MgCl2, 22-26% PEG MME-550

National Institutes of Health/National Cancer Institute (NIH/NCI)

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

cyclooxygenase, indomethacin, OXIDOREDUCTASE-OXIDOREDUCTASE INHIBITOR complex