T4Dam in Complex with AdoHcy and 13-mer Oligonucleotide Making Non- and Semi-specific (~1/4) Contact

Transition from Nonspecific to Specific DNA Interactions along the Substrate-Recognition Pathway of Dam Methyltransferase.

Structure of the bacteriophage T4 DNA adenine methyltransferase

Deoxyadenosyl-methyltransferase, M.EcoT4Dam

PEG MME 5000, citrate-phosphate, ammonium sulphate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K

Ternary Structure Of T4Dam With Adohcy and DNA

SEQUENCE

1) Author states that Q139R, Y140F and Q209L reflect
conflicts between deposited protein sequence and
translated deposited DNA-->protein sequence. From their
electron density, it appears the translated DNA sequence
is correct.
2) Residue 119 were modeled (for both A and B) as Tyr in
this structure as the electron density does not support
an Asp here. The electron denstiy for B119 fits a Tyr
residue perfectly.

DNA adenine methylase (EC 2.1.1.72)/DNA Complex

T4DAM, METHYLTRANSFERASE, DNA, Transferase-DNA COMPLEX