XFEL structure of the Soluble methane monooxygenase hydroxylase and regulatory subunit complex, from Methylosinus trichosporium OB3b, diferric state

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

High-Resolution XFEL Structure of the Soluble Methane Monooxygenase Hydroxylase Complex with its Regulatory Component at Ambient Temperature in Two Oxidation States.

Methane monooxygenase component A alpha chain

Methane monooxygenase regulatory protein B

30 mM Sodium-Iodide,
30 mM Sodium-Bromide,
30 mM Sodium-Fluoride,
20% (v/v) Glycerol,
10% (w/v) PEG 4000,
100 mM HEPES/MOPS pH 7.5
10 mM Iron(III) chloride

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

Biotechnology and Biological Sciences Research Council (BBSRC)

Department of Energy (DOE, United States)

drop on tape combined with acustic droplet ejection

Methane monooxygenase component A beta chain,Protein A beta subunit of soluble methane monooxygenase (E.C.1.14.13.25), Methane monooxygenase component A alpha chain (E.C.1.14.13.25), Methane monooxygenase component A gamma chain (E.C.1.14.13.25), Methane monooxygenase regulatory protein B

Ferritin superfamily, Soluble methane monooxygenase, Di-iron oxygen activation, Substrate oxidation., OXIDOREDUCTASE