SdnG, a Diels Alderase catalyzed the formation of norbornene skeleton in Sordarin biosynthetic pathway

Biosynthesis of Sordarin Revealing a Diels-Alderase for the Formation of the Norbornene Skeleton.

Sordarin/hypoxysordarin biosynthesis cluster protein G

Escherichia coli 'BL21-Gold(DE3)pLysS AG'

0.2 M Ammonium di-hydrogen phosphate, 0.1 M Tris pH 8.5 and 50 % v/v 2-Methyl-2,4-pentanediol

National Natural Science Foundation of China (NSFC)

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY

pericyclase, Diel Alderase, BIOSYNTHETIC PROTEIN