GH5-4 broad specificity endoglucanase from Ruminococcus flavefaciens

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

A structural and kinetic survey of GH5_4 endoglucanases reveals determinants of broad substrate specificity and opportunities for biomass hydrolysis.

1 microliter of protein at XXXX was combined with 1 microliter of reservoir solution consisting of 31%w/v PEG3350, 0.2 M NaCl, 0.1M bistris pH 5.5. Sample was cryoproected by brief exposure to ethanol vapor.

Department of Energy (DOE, United States)

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
NIH 5 T32 GM008349 Biotechnology Training Program

Cellulase, xylanase, manganese, GH5, endoglucanase, HYDROLASE