Structural and mechanistic studies on a cephalosporin esterase from the clavulanic acid biosynthesis pathway

(2R,3Z,5R)-3-(2-HYDROXYETHYLIDENE)-7-OXO-4-OXA-1-AZABICYCLO[3.2.0]HEPTANE-2-CARBOXYLIC ACID

Structural and mechanistic studies of the orf12 gene product from the clavulanic acid biosynthesis pathway.

13%(W/V) PEG 4000,0.1M NAAC, 0.1M NACL,10%(W/V) GLYCEROL,0.1M TRIS/HCL PH 8

ENGINEERED RESIDUE IN CHAIN A, SER 173 TO ALA ENGINEERED RESIDUE IN CHAIN B, SER 173 TO ALA
POTASSIUM CLAVULANATE (J01): 2 MOLECULES OF CLAVULANIC ACID ARE BOUND PER SUBUNIT

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. RESIDUES A1-A7,A44-A46,A435-A458,B1-B7 B436-A458 ARE DISORDERED