Crystal structure of Barley Beta-Amylase complexed with acarbose

Chemical Genetics and Cereal Starch Metabolism: Structural Basis of the Non-Covalent and Covalent Inhibition of Barley Beta-Amylase.

CRYSTALS WERE GROWN AT 291 K USING THE HANGING DROP VAPOUR DIFFUSION METHOD WITH PROTEIN AT 10 MG PER ML AND A PRECIPITANT COMPRISED OF 14 PERCENT PEG 3350 IN 100 MM BIS-TRIS PROPANE BUFFER AT PH 5.5

CRYSTAL STRUCTURE OF BARLEY BETA-AMYLASE COMPLEXED WITH 4-O-ALPHA-D- GLUCOPYRANOSYLMORANOLINE

CRYSTAL STRUCTURE OF BARLEY BETA-AMYLASE COMPLEXED WITH ALPHA-CYCLODEXTRIN

CRYSTAL STRUCTURE OF BARLEY BETA-AMYLASE COMPLEXED WITH 2,3-EPOXYPROPYL-ALPHA-D- GLUCOPYRANOSIDE

CRYSTAL STRUCTURE OF BARLEY BETA-AMYLASE COMPLEXED WITH 3,4-EPOXYBUTYL ALPHA-D- GLUCOPYRANOSIDE

TOWARDS THE DISSECTION OF CEREAL STARCH METABOLISM THROUGH CHEMICAL GENETICS: STRUCTURAL BASIS OF THE NON-COVALENT AND COVALENT INHIBITION OF BARLEY BETA-AMYLASE

DETERMINATION METHOD: DSSP
THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY
A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

1,2-ETHANEDIOL (EDO): PRESENT AT 20 PERCENT IN THE
CRYOPROTECTANT
BETA-ACARBOSE (QPS): SOAKED INTO CRYSTALS AT 10 MM FOR 1
HOUR

PROTEIN ISOLATED FROM NATURAL SOURCE BUT SEQUENCE DIFFERS AT 3 POSITIONS TO UNIPROTKB DATABASE ENTRY P16098. THESE CHANGES WERE IDENTIFIED FROM ELECTRON DENSITY MAPS.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.

HYDROLASE, CARBOHYDRATE METABOLISM, GLYCOSYL HYDROLASE FAMILY 14, STARCH DEGRADATION, GERMINATION