Avi-GCPII structure in complex with FITC-conjugated GCPII-specific inhibitor

N-({(1S)-5-[(4-bromobenzyl)({6-[4-(4-{4-[4-carboxy-3-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoyl]piperazin-1-yl}phenyl)piperazin-1-yl]pyridin-3-yl}carbonyl)amino]-1-carboxypentyl}carbamoyl)-L-glutamic acid

Design of highly potent urea-based, exosite-binding inhibitors selective for glutamate carboxypeptidase II.

avi-tagged extracellular portion, UNP residues 44-750

Cell growth-inhibiting gene 27 protein,Folate hydrolase 1,Folylpoly-gamma-glutamate carboxypeptidase,FGCP,Glutamate carboxypeptidase II,GCPII,Membrane glutamate carboxypeptidase,mGCP,N-acetylated-alpha-linked acidic dipeptidase I,NAALADase I,Prostate-specific membrane antigen,PSMA,Pteroylpoly-gamma-glutamate carboxypeptidase

33% pentaerythritol propoxylate
(Hampton Research), 1.5% PEG 3350 (Fluka), 100 mM Tris HCl

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED

avi-tagged extracellular portion of glutamate carboxypeptidase II (E.C.3.4.17.21)

glycoprotein, metallopeptidase, inhibitor, hydrolase