Time-Resolved Crystal Structure of HindIII with 0sec soaking

Analysis of the HindIII-catalyzed reaction by time-resolved crystallography

This DNA sequence synthesized chemically contains cognate HindIIIR recognition sequence and elongated scaffolds on both side
DNA (5'-D(*GP*CP*CP*AP*AP*GP*CP*TP*TP*GP*GP*C)-3')

R.HindIII, Endonuclease HindIII, Type II restriction enzyme HindIII

10-15% PEG 3350, 300mM NaSCN, 10% Glycerol, 100mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES: REFINED INDIVIDUALLY

Type-2 restriction enzyme HindIII (E.C.3.1.21.4)/DNA

FREEZE-TRAP, time-resolved, type IIP restriction endonuclease, restriction endonuclease, hydrolase, nuclease, HYDROLASE-DNA complex