Crystal structure of an arabinose binding protein with designed serotonin binding site in open, ligand-free state

The Computational Design of Ligand Binding is not a Solved Problem

20% PEG 3350, 0.2M (NH4)CITRATE, 2UMOL/ML SEROTONIN, pH 8.0

ENGINEERED RESIDUE IN CHAIN A, LYS 33 TO ALA ENGINEERED RESIDUE IN CHAIN A, TRP 39 TO ALA ENGINEERED RESIDUE IN CHAIN A, PHE 40 TO SER ENGINEERED RESIDUE IN CHAIN A, GLU 43 TO GLN ENGINEERED RESIDUE IN CHAIN A, CYS 87 TO SER ENGINEERED RESIDUE IN CHAIN A, ASP 113 TO ASN ENGINEERED RESIDUE IN CHAIN A, MET 131 TO GLU ENGINEERED RESIDUE IN CHAIN A, ASN 200 TO GLU ENGINEERED RESIDUE IN CHAIN A, LEU 321 TO CYS ENGINEERED RESIDUE IN CHAIN B, LYS 33 TO ALA ENGINEERED RESIDUE IN CHAIN B, TRP 39 TO ALA ENGINEERED RESIDUE IN CHAIN B, PHE 40 TO SER ENGINEERED RESIDUE IN CHAIN B, GLU 43 TO GLN ENGINEERED RESIDUE IN CHAIN B, CYS 87 TO SER ENGINEERED RESIDUE IN CHAIN B, ASP 113 TO ASN ENGINEERED RESIDUE IN CHAIN B, MET 131 TO GLU ENGINEERED RESIDUE IN CHAIN B, ASN 200 TO GLU ENGINEERED RESIDUE IN CHAIN B, LEU 321 TO CYS

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.

SUGAR TRANSPORT, ARABINOSE BINDING PROTEIN, PERIPLASMIC BINDING PROTEIN, ABP, TRANSPORT PROTEIN, PERIPLASM, RECEPTOR DESIGN