D-threo-3-hydroxyaspartate dehydratase from Delftia sp. HT23 complexed with D-erythro-3-hydroxyaspartate

Structural insights into the substrate stereospecificity of D-threo-3-hydroxyaspartate dehydratase from Delftia sp. HT23: a useful enzyme for the synthesis of optically pure L-threo- and D-erythro-3-hydroxyaspartate

D-THA DH, D-THA dehydratase, Threo-3-hydroxy-D-aspartate ammonia-lyase

12% PEG3350, 0.2M MgCl2, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

wild-type enzyme complexed with D-allothreonine

SG ATOM OF CYS 353 AND NE2 ATOM OF HIS 351 ARE COORDINATED TO THE ACTIVE-SITE METAL MG.

HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT

D-threo-3-hydroxyaspartate dehydratase (E.C.4.3.1.27)