Crystal Structure Analysis of Plant Exohydrolase

Discovery of processive catalysis by an exo-hydrolase with a pocket-shaped active site.

Beta-D-glucan exohydrolase isoenzyme ExoI

75mM HEPES-NaOH pH7.0, 1.2% PEG 400, 1.7M ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 277K

THE AUTHORS STATE THERE IS AN ERROR IN THE CDNA SEQUENCING OF AF102868 (GENBANK ACCESSION NUMBER). RESIDUE 320 (SEQUENCE DATABASE RESIDUE 345) IS LYS AND IS NOT ASN.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Beta-D-glucan exohydrolase isoenzyme ExoI (E.C.3.2.1.-)

BETA BARREL, HYDROLASE, GRAIN DEVELOPMENT, Tim Barrel/Beta sheet, N-glycosylation, plant apoplast, Enzyme Function Initiative