Crystal structure of polyneuridine aldehyde esterase mutant (H244A)

Structural Basis and Enzymatic Mechanism of the Biosynthesis of C9- from C10-Monoterpenoid Indole Alkaloids.

0.2 M BIS-TRIS, 0.25 M LI2SO4, 25% POLYETHYLENE GLYCOL 3350, PH 6.30

CRYSTAL STRUCTURE OF POLYNEURIDINE ALDEHYDE ESTERASE

ENGINEERED RESIDUE IN CHAIN A, HIS 244 TO ALA ENGINEERED RESIDUE IN CHAIN B, HIS 244 TO ALA ENGINEERED RESIDUE IN CHAIN C, HIS 244 TO ALA ENGINEERED RESIDUE IN CHAIN D, HIS 244 TO ALA ENGINEERED RESIDUE IN CHAIN E, HIS 244 TO ALA

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. ATOM RECORD CONTAINS RESIDUAL B FACTORS ONLY.

POLYNEURIDINE ALDEHYDE ESTERASE (E.C.3.1.1.78)

ALKALOID METABOLISM, MONOTERPENOID INDOLE ALKALOIDS, PNAE, HYDROLASE, SERINE ESTERASE