Structure of TAB5 alkaline phosphatase mutant His 135 Asp with Zn bound in the M3 site.

Coordination Sphere of the Third Metal Site is Essential to the Activity and Metal Selectivity of Alkaline Phosphatases.

PHOSPHOSERINE RESIDUE AT POSITION 84. M1, M2, M3 OCCUPIED BY ZN.

20.00MM TRIS-CL PH8.0, 10.00MM MGCL2, 0.01MM ZNCL2, 23.00% PEG 3350, 0.20MM SODIUM ACETATE, 0.10MM SODIUM CACODYLATE, PH 6.5

STRUCTURE OF ALKALINE PHOSPHATASE FROM THE ANTARCTIC BACTERIUM TAB5

STRUCTURE OF TAB5 ALKALINE PHOSPHATASE MUTANT HIS 135 ASP WITH MG BOUND IN THE M3 SITE.

STRUCTURE OF TAB5 ALKALINE PHOSPHATASE MUTANT HIS 135 GLU WITH MG BOUND IN THE M3 SITE.

ENGINEERED RESIDUE IN CHAIN A, HIS 135 TO ASP ENGINEERED RESIDUE IN CHAIN B, HIS 135 TO ASP

PSYCHROPHILES, COLD ADAPTATION, ALKALINE PHOSPHATASE, HYDROLASE