CryoEM structure of rhinovirus B14 in complex with C5 Fab (33 degrees Celsius, molar ratio 1:3, empty particle)

Antibody-induced uncoating of human rhinovirus B14.

A combination of the following approaches was used: (1) model rebuilding using Coot, (2) real space refinement using Phenix, (3) reciprocal space refinment using Phenix (as in standard crystallographic refinement).

20 mM Tris, 120 mM sodium chloride, 1 mM EDTA, pH 8.0

On-the-fly CTF correction was performed during 2D alignment and 3D reconstruction.

Viruses were grown in HeLa-H1 cells. Full virions were converted into empty particles upon binding to Fab fragments.

National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)

CryoEM structure of rhinovirus B14 in complex with C5 Fab (33 degrees Celsius, molar ratio 1:3, full particle)

CryoEM structure of rhinovirus B14 in complex with C5 Fab (4 degrees Celsius, molar ratio 1:3, full particle)

CryoEM structure of rhinovirus B14 in complex with C5 Fab (33 degrees Celsius, molar ratio 1:1, full particle)

icosahedral asymmetric unit, std point frame

Based on previously published structures

C5 antibody variable heavy domain, C5 antibody variable light domain, viral protein 1, viral protein 3, viral protein 2

virus, antibody, VIRUS LIKE PARTICLE-IMMUNE SYSTEM complex