A Dodecameric Thioferritin in the Bacterial Domain, Characterization of the Bacterioferritin-Related Protein from Bacteroides fragilis

Characterization of the Bacteroides Fragilis Bfr Gene Product Identifies a Bacterial Dps-Like Protein and Suggests Evolutionary Links in the Ferritin Superfamily.

PUTATIVE BACTERIOFERRITIN-RELATED PROTEIN

0.1 M BIS-TRIS HCL PH 8.5, 0.25 M MGCL2, 16 % (W/V) PEG 4,000, 2 % (W/V) BENZAMIDINE HCL, 20 % GLYCEROL

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. SOLVENT MOLECULES IN THE VICINITY OF X=- 18.9, Y=89.5, Z=10.3 AND X=-1.8, Y=96.5, Z=-5.4 ARE ASSOCIATED WITH RESIDUAL ELECTRON DENSITY A BIS-TRIS BUFFER MOLECULE, USED IN PROTEIN PURIFICATION, MAY OCCUPY EACH OF THESE POSITIONS INSTEAD OF SOME SOLVENT MOLECULES.

DPS, DPSL, IRON, FERRITIN, OXIDATIVE STRESS, METAL TRANSPORT