Crystal structure of the WbkC N-formyltransferase (F142A variant) from Brucella melitensis

N-{[4-({[(6R)-2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)phenyl]carbonyl}-L-glutamic acid
[6R]-5-FORMYL-5,6,7,8-TETRAHYDROFOLATE; 6R-FOLINIC ACID

Biochemical Characterization of WbkC, an N-Formyltransferase from Brucella melitensis.

Gdp-mannose 4,6-dehydratase / gdp-4-amino-4,6-dideoxy-d-mannose formyltransferase

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)

13-15% PEG5000, 2% glycerol, 5 mM GDP-perosamine, 5 mM folinic acid, 100 mM MES

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Gdp-mannose 4,6-dehydratase / gdp-4-amino-4,6-dideoxy-d-mannose formyltransferase (E.C.2.1.2.-,4.2.1.47)