Glutamate Racemase (MurI) from E. faecalis in complex with a 9-Benzyl Purine inhibitor

Exploring 9-Benzyl Purines as Inhibitors of Glutamate Racemase (Muri) in Gram-Positive Bacteria.

PROTEIN FORMULATED AT 10 MG/ML WITH 200MM AMMONIUM ACETATE PH 7.4, 5MM D-L GLUTAMATE, 1 MM TCEP AND CRYSTALLISED WITH 0.2 MM MGCL2 AND 20-25% PEG 3350, 100 MM TRIS PH 7.5, 20% GLYCEROL

CRYSTAL STRUCTURE OF ENTEROCOCCUS FAECALIS GLUTAMATE RACEMASE IN COMPLEX WITH D- GLUTAMATE

CRYSTAL STRUCTURE OF ENTEROCOCCUS FAECALIS GLUTAMATE RACEMASE IN COMPLEX WITH D- AND L-GLUTAMATE

DATABASE REFERENCE GENESEQP ADR04180 (PATENT DATABASE.) CLOSEST PUBLIC REFERENCE IS GENPEPT NP_814851 HAS 2 DIFFERENCES AT RESIDUE 137 AND 144 (THR AND ASP IN NP_814851)

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.

ISOMERASE, GLUTAMATE RACEMASE, PEPTIDOGLYCAN SYNTHESIS, CELL WALL BIOGENESIS/DEGRADATION, CELL SHAPE, BENZYL PURINE, MURI INHIBITOR