The Structure of an Open Form of an E. Coli Mechanosensitive Channel at 3.45 A Resolution.

SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL

THE BEST CRYSTALS (BY VISUAL INSPECTION) WERE OBTAINED USING 0.1M HEPES PH 7.2, 0.2 M SODIUM FORMATE, 14 % PEG3350, 8 % GLYCEROL AS PRECIPITANT. PRIOR TO DATA COLLECTION, CRYSTALS WERE TRANSFERRED INTO A SOLUTION CONTAINING 40MM TRIS PH 7.5, 0.01% FOS-CHOLINE-14, 0.15 M NACL, 0.3 M SODIUM FORMATE, 20% GLYCEROL AND 22% PEG3350.

SHEET
DETERMINATION METHOD: DSSP
THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 34-STRANDED BARREL THIS IS REPRESENTED BY
A 35-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "AC" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 6-STRANDED BARREL THIS IS REPRESENTED BY
A 7-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

ENGINEERED RESIDUE IN CHAIN A, ALA 106 TO VAL ENGINEERED RESIDUE IN CHAIN B, ALA 106 TO VAL ENGINEERED RESIDUE IN CHAIN C, ALA 106 TO VAL ENGINEERED RESIDUE IN CHAIN D, ALA 106 TO VAL ENGINEERED RESIDUE IN CHAIN E, ALA 106 TO VAL ENGINEERED RESIDUE IN CHAIN F, ALA 106 TO VAL ENGINEERED RESIDUE IN CHAIN G, ALA 106 TO VAL

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.

ION TRANSPORT, TRANSMEMBRANE, INNER MEMBRANE, MEMBRANE STRUCTURE, MEMBRANE PROTEIN, MEMBRANE, TRANSPORT, OPEN CHANNEL, IONIC CHANNEL