Structure of the SpCas9 DNA adenine base editor - ABE8e

DNA capture by a CRISPR-Cas9-guided adenine base editor.

20 mM Tris-HCL (pH 7.5)
200 mM KCl
1 mM DTT
0.25% (v/v) Glycerol

ABE8e in a substrate-bound state where the deaminase (TadA-8e) engages DNA exposed within the CRISPR-Cas9 R-loop complex.
CRISPR-Cas9 ABE8e in a substrate-bound state

The grids were rapidly plunged into liquid ethane using a FEI Vitrobot MarkIV maintained at 8C and 100% humidity, after being blotted for 4.5s with blot force 8.

National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)

Base editor, ABE, Cas9, CRISPR, DNA BINDING PROTEIN-DNA-RNA complex