CryoEM model of CopA, the copper transporting ATPase from Archaeoglobus fulgidus

Structure of a Copper Pump Suggests a Regulatory Role for its Metal-Binding Domain.

METHOD--MANUAL REFINEMENT PROTOCOL--X-RAY

ALL AVERAGING DONE IN FOURIER SPACE. ALL TUBES AVERAGED HAD IDENTICAL HELICAL SYMMETRY
TUBES DIVIDED INTO THIRDS, QUARTERS, OR FIFTHS AND CORRECTED FOR IN-PLACE ROTATION, OUT-OF-PLANE TILT, Z-SHIFT, ROTATION ABOUT Z-AXIS

50 MM MES PH 6.1 25 MM NA2SO4 25 MM K2SO4 10 MM MGSO4 2 MM 2-MERCAPTOETHANOL 0.2 MM BCDS

MICROGRAPHS SCANNED AT 14 MICRON INTERVAL USING ZEISS- SCAI SCANNER

LIQUID ETHANE. SAMPLES FROZEN IN COLD ROOM

SARCOPLASMIC/ENDOPLASMIC RETICULUM CALCIUM ATPASE 1

EM MAP TO WHICH THIS SEQUENCE WAS MODELED
PDB ENTRIES USED TO MODEL CHAIN A: 1JWW
PDB ENTRIES USED TO MODEL CHAINS B, C, D, E, G, H, K, L: 1WPG
PDB ENTRIES USED TO MODEL CHAIN F: 2HC8
PDB ENTRIES USED TO MODEL CHAINS I, J: 2B8E

HYDROLASEP-TYPE ATPASE, CRYO-EM, HELICAL RECONSTRUCTION, MEMBRANE PROTEIN, COPPER TRANSPORTER, METAL BINDING DOMAIN, HYDROLASE