Crystal structure of enoyl-CoA hydratase/isomerase from Pseudoalteromonas atlantica T6c at 2.3 A resolution.

New York Structural Genomics Research Consortium (NYSGRC)

Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)

0.2 ul of 20 mg/ml protein in 20 mM HEPES pH 7.5, 150 mM NaCl, 10% Glycerol, 0.1% Sodium Azide and 0.5 mM TCEP were mixed with 0.2 ul of the JCSG+ condition #89 (0.2M ammonium acetate, 0.1M Bis-Tris pH 5.5, 45% (v/v) MPD) and equilibrated against 0.9 M NaCl solution in 96 Well 3 drop Crystallization Plate (SwissCi).

New York Structural Genomics Research Consortium

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

U VALUES : WITH TLS ADDED HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
software from EMBL (with LS-CAT developed extensions)
Structural Genomics, PSI-Biology, New York Structural Genomics Research Consortium, NYSGRC, ISOMERASE, LYASE