Crystal structure of the O-carbamoyltransferase TobZ K443A variant in complex with ATP, ADP and carbamoyl phosphate

The O-Carbamoyltransferase TobZ Catalyzes an Ancient Enzymatic Reaction.

1.6 M sodium potassium tartrate, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K

TobZ in complex with carbamoyl phosphate

TobZ in complex with carbamoyl adenylate intermediate

TobZ in complex with AMPCPP and carbamoyl phosphate

TobZ in complex with Tobramycin, carbamoyl phosphate and ADP

TobZ H14N variant in complex with carbamoyl adenylate intermediate

TobZ M473I variant in complex with carbamoyl phosphate and ADP

TobZ S530A variant in complex with carbamoyl phosphate and ADP

O-carbamoyltransferase TobZ (E.C.2.1.3.-)

antibiotic biosynthesis, substrate assisted catalysis, substrate channeling, adenylation, structural enzymology, enzyme evolution, TRANSFERASE