Glucokinase in complex with an activator and glucose

Insights into Mechanism of Glucokinase Activation: OBSERVATION OF MULTIPLE DISTINCT PROTEIN CONFORMATIONS.

Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D

Protein was in 25 mM HEPES pH 7.0, 0.5 mM TCEP, 0.05 M NaCl, 40 mM glucose at 20 mg/mL and 1 mM a GKA. Crystallization drops in a 1:1 ratio were set up over wells containing 0.1 M Tris HCl, pH 7.0, 80-200 mM glucose, and 19-26% PEG-4000, VAPOR DIFFUSION, HANGING DROP

catalysis reaction, TRANSFERASE, TRANSFERASE-TRANSFERASE ACTIVATOR complex