T.thermophilus 30S ribosome with KsgA, class K2

Decoding the Mechanism of Specific RNA Targeting by Ribosomal Methyltransferases.

Phenix was used in real space for initial refinement. The final refinements was performed with REFMAC within ccpem

30S ribosomal subunit purified from delta KsgA strain and KsgA recombinantly purified has been added after isolation for complex formation
30S ribosomal with bound KsgA, a RNA methyltransferase

Total number of frames is 30. Each frame had 1.43 e/A2

~2 micromolar concentration of ribosome+KsgA was used during freezing

After application of the complex, 15 seconds wait time was given before blotting.

Ribosomal RNA small subunit methyltransferase A

16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase,16S rRNA dimethyladenosine transferase,16S rRNA dimethylase,S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase

Department of Biotechnology (DBT, India)
Science and Engineering Research Board (SERB)
30S subunit, KsgA, rRNA methyltransferase, RIBOSOME