LarE, a sulfur transferase involved in synthesis of the cofactor for lactate racemase in complex with cadmium

Crystallographic characterization of a tri-Asp metal-binding site at the three-fold symmetry axis of LarE.

ATP-dependent sacrificial sulfur transferase LarE

ATP-utilizing enzyme of the PP-loopsuperfamily,Lactate racemization operon protein LarE,PP-loop superfamily ATP-binding protein,TIGR00268 family protein

5 uL ~25 mg/ml LarE (100 mM Tris-HCl pH 7.5, 300 mM NaCl) mixed with 5 uL of reservoir solution. Hanging drop reservoir contained 100 uL of 30% v/v pentaerythritol ethoxylate (15/4 EO/OH), 50 mM BIS-TRIS pH 6.5, 100 mM ammonium sulfate. Crystal soaked 60 minutes in 3.8 mM cadmium chloride, 30% v/v pentaerythritol ethoxylate (15/4 EO/OH), 50 mM BIS-TRIS pH 6.5, 50 mM ammonium sulfate

National Science Foundation (NSF, United States)

Lar, sulfur transferase, LarE, AMPylation, PP-loop, ATP pyrophophatase domain, lactate racemization, lactate racemase, TRANSFERASE

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