Crystal structure of R421A variant of cytosolic fumarate hydratase from Leishmania major in a complex with S-malate

Structural and Biochemical Investigations of the [4Fe-4S] Cluster-Containing Fumarate Hydratase fromLeishmania major.

12% v/v PEG3350, 10 mM ammonium citrate tribasic, 16 mM sodium acetate trihydrate, 20 mM sodium formate, 6.4 mM ammonium tartrate dibasic, 12 mM S-malate

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

fumarate hydratase, fumarase, Leishmania major, substrate, variant R421A, S-malate, LYASE

(chain A and (resid 28 through 54 or (resid 55 and (name N or name CA or name C or name O or name CB or name CG )) or resid 56 through 60 or (resid 61 and (name N or name CA or name C or name O or name CB or name CG )) or resid 62 through 65 or (resid 66 through 67 and (name N or name CA or name C or name O or name CB )) or resid 68 through 79 or resid 81 through 83 or resid 85 through 91 or (resid 92 and (name N or name CA or name C or name O or name CB )) or resid 93 through 127 or resid 129 through 196 or resid 198 through 233 or (resid 234 and (name N or name CA or name C or name O or name CB )) or resid 235 through 301 or (resid 302 and (name N or name CA or name C or name O or name CB )) or resid 303 through 368 or (resid 369 and (name N or name CA or name C or name O or name CB )) or resid 370 through 375 or resid 386 through 387 or (resid 388 and (name N or name CA or name C or name O or name CB )) or resid 389 through 396 or (resid 397 and (name N or name CA or name C or name O or name CB )) or resid 398 through 409 or resid 411 through 466 or resid 468 through 480 or resid 482 through 495 or (resid 496 and (name N or name CA or name C or name O or name CB )) or resid 497 through 521 or (resid 522 and (name N or name CA or name C or name O or name CB or name CG )) or resid 523 through 537 or resid 539 through 542 or (resid 543 and (name N or name CA or name C or name O or name CB or name CG or name CD )) or resid 544 through 568))

(chain B and (resid 28 through 43 or (resid 44 and (name N or name CA or name C or name O or name CB or name CG )) or resid 45 through 50 or (resid 51 and (name N or name CA or name C or name O or name CB )) or resid 52 through 79 or resid 81 through 83 or resid 85 through 119 or (resid 120 and (name N or name CA or name C or name O or name CB or name CG or name CD )) or resid 121 through 127 or resid 129 through 183 or (resid 184 and (name N or name CA or name C or name O or name CB or name CG )) or resid 185 through 196 or resid 198 through 409 or resid 411 through 466 or resid 468 through 480 or resid 482 through 537 or resid 539 through 568))