Crystal structure of Cas9 bound to PAM-containing DNA target with mismatches at positions 1-2

Structural Basis of Pam-Dependent Target DNA Recognition by the Cas9 Endonuclease

CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1

0.1 M TRIS-ACETATE, PH 8.5, 0.3 M KSCN, 15% (W/V) PEG 3350

CRYSTAL STRUCTURE OF CAS9 BOUND TO PAM-CONTAINING DNA TARGET

CRYSTAL STRUCTURE OF CAS9 BOUND TO PAM-CONTAINING DNA TARGET CONTAINING MISMATCHES AT POSITIONS 1-3

N-TERMINAL GAAS SEQUENCE IS DERIVED FROM EXPRESSION PLASMID. H840A MUTATION WAS INTRODUCED TO INACTIVATE THE HNH DOMAIN. D10A MUTATION WAS INTRODUCED TO INACTIVATE THE RUVC DOMAIN.

CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1/RNA Complex

HYDROLASE-DNA-RNA COMPLEX, GENOME EDITING, RNP, PROTEIN-RNA COMPLEX