CryoEM structure of an influenza virus receptor-binding site antibody-antigen interface - Class 2

CryoEM Structure of an Influenza Virus Receptor-Binding Site Antibody-Antigen Interface.

Domains were initially placed manually using Chimera. The HA trimer was from PDB ID 5UGY. Fab was initially obtained using MODELLER, with PDB ID 4K8R as template. The structure was refined using PHENIX.

Refinement and classification were limited to 10 Angstrom resolution.

Beta-octylglucoside was added to a final concentration of 0.07% w/v to induce more variable particle orientations.

The complex consists of three hemagglutinin head domains bound to three single-chain variable-domain fragments.
Influenza-virus hemagglutinin H1 complexed with K1915 single-chain variable-domain fragment

Influenza A virus (A/Solomon Islands/3/2006(H1N1))

The particle images were normalized to have constant variance and zero average. Movies were processed using Unblur.

The exposure rate was 8 electrons/physical pixel/second.

National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)

Hemagglutinin HA1, Hemagglutinin HA2, scFV

viral glycoprotein, hemagglutinin, antibody fragment, VIRAL PROTEIN-IMMUNE SYSTEM complex