Cytochrome P411 P-4 A82L A78V F263L amination catalyst

Enantioselective, intermolecular benzylic C-H amination catalysed by an engineered iron-haem enzyme.

Due to the extensive engineering performed to obtain this catalyst, We created a simplified name for the catalyst. The mutation details are explained in the publication

0.1 M Bis-tris pH 5.0, 13% PEG 3350, 0.2 M NaHCOO, 22 mg/ml protein

National Science Foundation (NSF, United States)

National Institutes of Health/National Center for Research Resources (NIH/NCRR)

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

engineered, C-H functionalization, nitrene transfer, P411CHA, OXIDOREDUCTASE