Magic Angle Spinning NMR Structure of Human Cofilin-2 Assembled on Actin Filaments

Magic Angle Spinning NMR Atomic-Resolution Structure of Human Cofilin-2 Assembled on Actin Filaments Reveals Isoform-Specific Conformation and Binding Mode

National Science Foundation (NSF, United States)

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

20.8 % w/w [U-13C; U-15N] human cofilin-2, 79.2 % w/w F-actin, Solid
Uniformly labeled human cofilin-2 in complex with natural abundance actin filaments obtained using rabbit skeletal muscle actin

20.8 % w/w [1,6-13C]-glucose, U-15N human cofilin-2, 79.2 % w/w F-actin, Solid
Selectively labeled human cofilin-2 in complex with natural abundance actin filaments obtained using rabbit skeletal muscle actin

20.8 % w/w [2-13C]-glucose, U-15N human cofilin-2, 79.2 % w/w F-actin, Solid

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax

Schwieters, Kuszewski, Tjandra and Clore

actin filament binding, actin filament severing, cytoskeletal assembly, CONTRACTILE PROTEIN, CELL CYCLE