CRYSTAL STRUCTURE OF A SELF-SPLICING GROUP I INTRON WITH BOTH EXONS

ADENOSINE-5'-PHOSPHATE-2',3'-CYCLIC PHOSPHATE

Crystal Structure of a Group I Intron Splicing Intermediate

RNA Kink Turns to the Left and to the Right

In Vitro Splicing of the Ribosomal RNA Precursor of Tetrahymena: Involvement of a Guanosine Nucleotide in the Excision of Intervening Sequence

Self-Splicing Intron in tRNA Genes of Widely Divergent Bacteria

5'-R(*AP*AP*GP*CP*CP*AP*CP*AP*CP*AP*AP*AP*CP*CP*AP*GP*AP*CP*GP *GP*CP*C)-3'

SEQUENCE
NUCLEOTIDES 1001B TO 1014B BELONG TO AN ENGINEERED U1A LOOP AND ARE INSERTED BETWEEN GUA-107B AND CYT-112B. THERE ARE NO NUCLEOTIDES BETWEEN GUA-1B AND GUA-5B DUE TO A CLONING DESIGN. O2' OF DEOXY MODIFICATION OF RNA NUCLEOTIDES ARE INCLUDED WITH Q=0.00 AND B=0.00. THEY ARE AT GUA-206C, CYT-1C, CYT-205C, AND THY-1D. CHAINS B AND C ARE TWO PARTS OF THE ORIGINAL ONE INTRON SEQUENCE BECAUSE OF AN EXPERIMENTAL DESIGN.
THE U1A PROTEIN USED IN THIS STUDY WAS A DOUBLE MUTANT IN WHICH TYR-31/GLN-36 WAS REPLACED WITH HIS-31/ARG-36.
THE FIRST THREE RESIDUES IN U1A PROTEIN WERE MISSING IN THE STRUCTURE.

RNA WAS IS TRANSCRIBED BY T7 RNA POLYMERASE.

INTRON, EXON, RIBOZYME, GROUP I, U1A, RNA, STRUCTURAL PROTEIN-RNA COMPLEX