Crystal structure at 2.4 angstroms resolution of the complex of transducin betagamma and its regulator, phosducin.

Crystal Structure of a Ga Protein Beta Gamma Dimer at 2.1A Resolution

LYS-C RESISTANT FRAGMENT, THE GAMMA SUBUNIT CLEAVED AFTER RESIDUE 68

THE PROTEIN COMPLEX (10 MG/ML SOLUTION) WAS CRYSTALLIZED FROM 50 MM SODIUM CITRATE (PH 5.0), 150 MM MAGNESIUM ACETATE, 9.5% PEG 8000, BY HANGING DROP METHOD AT 4 DEGREES C., vapor diffusion - hanging drop, temperature 277K

DISORDERED REGION IN PHOSDUCIN FROM RESIDUE 37 - 66 WAS MODELED STEREOCHEMICALLY AS A POLYALANINE CHAIN.

PHOSDUCIN, TRANSDUCIN, BETA-GAMMA, SIGNAL TRANSDUCTION, REGULATION, PHOSPHORYLATION, G PROTEINS, THIOREDOXIN, VISION, MEKA, COMPLEX (TRANSDUCER-TRANSDUCTION), COMPLEX (TRANSDUCER-TRANSDUCTION) complex