Camel nanobodies 7A3 and 8A2 broadly neutralize SARS-CoV-2 variants

Camel nanobodies broadly neutralize SARS-CoV-2 variants

Ternary complex of SARS-Cov-2 Spike protein ectodomain (Wuhan strain) and camel nanobodies 7A3 and 8A2.

Severe acute respiratory syndrome coronavirus 2

Complexes of spike with 7A3 and/or 8A2 VHHs were prepared by mixing the components at a spike trimer to nanobody molar ratio of 1:6. The final concentration of spike trimer was 3 uM in PBS at pH 7 with the addition of 5 mM imidazole. Because the 8A2 stock was too diluted, complexes involving this nanobody were prepared at 0.5 uM spike trimer followed by a 6-fold concentration using a 10 kDa cutoff centrifugal filter (Amicon Ultra). All complexes were incubated on ice for at least 5 min prior to grid preparation.

F817P, A892P, A899P, A942P, K986P, V987P, and residues 682-685 from RRAR to GSAS

National Institutes of Health/National Institute of Environmental Health Sciences (NIH/NIEHS)

National Institutes of Health/National Cancer Institute (NIH/NCI)

Neutralization, nanobody, VIRAL PROTEIN, VIRAL PROTEIN-IMMUNE SYSTEM complex