RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution

1-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Taking snapshots of photosynthetic water oxidation using femtosecond X-ray diffraction and spectroscopy.

Towards automated crystallographic structure refinement with phenix.refine.

PHENIX: a comprehensive Python-based system for macromolecular structure solution.

No monochromator, FEL beam with 20-30 eV bandwidth

Photosystem II core light harvesting protein

Photosystem II reaction center protein H

Photosystem II reaction center protein I

Photosystem II reaction center protein J

Photosystem II reaction center protein K

Photosystem II reaction center protein L

Photosystem II reaction center protein M

Photosystem II manganese-stabilizing polypeptide

Photosystem II reaction center protein T

Photosystem II reaction center protein Ycf12

Photosystem II reaction center X protein

Photosystem II reaction center protein Y

Photosystem II reaction center protein Z

32 kDa thylakoid membrane protein 1,Photosystem II protein D1 1

PSII D2 protein,Photosystem Q(A) protein

PS II complex 12 kDa extrinsic protein,PSII-U

Cytochrome c-549,Cytochrome c550,Low-potential cytochrome c

4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

Department of Energy (DOE, United States)

Photosystem II in the dark state at 4.9 A resolution

Photosystem II 500 ms after the third illumination (3F) at 4.6 A resolution

Photosystem II 500 ms after the second illumination (2F) at 4.5 A resolution

Molecule 19 (PHOTOSYSTEM II PROTEIN Y): THE DEPOSITORS STATE THAT THE SAMPLE SEQUENCE IS MDWRVLVVLLPVLLAAGWAVRNILPYAVKQVQKLLQKAKAA (UNP Q8DKM3).

Photosystem Q(B) protein 1 (E.C.1.10.3.9), Photosystem II core light harvesting protein, Photosystem II CP43 protein, Photosystem II D2 protein (E.C.1.10.3.9), Cytochrome b559 subunit alpha, Cytochrome b559 subunit beta, Photosystem II reaction center protein H, Photosystem II reaction center protein I, Photosystem II reaction center protein J, Photosystem II reaction center protein K, Photosystem II reaction center protein L, Photosystem II reaction center protein M, Photosystem II manganese-stabilizing polypeptide, Photosystem II reaction center protein T, Photosystem II 12 kDa extrinsic protein, Cytochrome c-550, Photosystem II reaction center protein ycf12, Photosystem II reaction center X protein, Photosystem II reaction center protein Y, Photosystem II reaction center protein Z
photosynthesis, water oxidation, membrane protein, X-ray free electron laser, ELECTRON TRANSPORT