THREE-DIMENSIONAL STRUCTURE OF THEILER VIRUS

SOLVENT MOLECULES WITH OCCUPANCIES SIGNIFICANTLY GREATER THAN 1.0 ARE SUSPECTED TO BE ANION OR CATION SITES.

Structural Factors that Control Conformational Transitions and Serotype Specificity in Type 3 Poliovirus

THEILER'S MURINE ENCEPHALOMYELITIS VIRUS (SUBUNIT VP1)

THEILER'S MURINE ENCEPHALOMYELITIS VIRUS (SUBUNIT VP2)

THEILER'S MURINE ENCEPHALOMYELITIS VIRUS (SUBUNIT VP3)

THEILER'S MURINE ENCEPHALOMYELITIS VIRUS (SUBUNIT VP4)

Theiler's encephalomyelitis virus (STRAIN DA)

THE ENTRY PRESENTED HERE DOES NOT CONTAIN THE COMPLETE
CRYSTAL ASYMMETRIC UNIT. IN ADDITION, THE COORDINATES
ARE NOT PRESENTED IN THE STANDARD CRYSTAL FRAME.

IN ORDER TO GENERATE THE FULL CRYSTAL AU, APPLY THE
FOLLOWING TRANSFORMATION MATRIX OR MATRICES AND SELECTED
BIOMT RECORDS TO THE COORDINATES, AS SHOWN BELOW.
X0 1 1.000000 0.000000 0.000000 0.00000
X0 2 0.000000 1.000000 0.000000 0.00000
X0 3 0.000000 0.000000 1.000000 0.00000
CRYSTAL AU =
(X0) * (BIOMT 1-5,11-15,21-30,41-50) * CHAINS 1,2,3,4

icosahedral asymmetric unit, std point frame

THE ELECTRON DENSITY WAS INCONSISTENT WITH THE PREDICTED AMINO ACID SEQUENCE DERIVED FROM THE GENE SEQUENCE OF THE DA STRAIN OF TMEV AT THREE SITES. THE STRUCTURE PRESENTED IN THIS ENTRY REFLECTS THE ELECTRON DENSITY AT THESE SITES. RESIDUE 214 OF VP1 WAS BUILT AS GLY. THIS RESIDUE HAS BEEN REPORTED TO BE EITHER LEU (Y.OHARA,S.STEIN,J.FU,L.STILLMAN, L.KLAMAN,R.P.ROOS (1988) VIROLOGY 164, 244-255) OR TRP (A.ZURBRIGGEN,J.M.HOGLE,R.S.FUJINAMI (1989) J.EXP.MED. 170, 2037-2049). SURROUNDING PORTIONS OF THE STRUCTURE PACK TOO CLOSELY TO PERMIT THIS RESIDUE TO HAVE A SIDE CHAIN AND NO SIDE CHAIN DENSITY IS VISIBLE IN THE MAP. RESIDUES 202 AND 230 OF VP3 ARE REPORTED TO BE ALA IN THE DATABASE BUT IN BOTH CASES THE ELECTRON DENSITY SUGGEST A LARGER SIDE CHAIN, EITHER THR OR VAL. A THR SIDE CHAIN WAS BUILT INTO POSITION 202 AND A VAL INTO POSITION 230. IN BOTH CASES THE CHANGE IN ASSIGNMENT IS CONSISTENT WITH THE LOCAL ENVIRONMENT OF THE SIDE CHAIN AND WITH THE SEQUENCE OF THE BEAN AND GDVII STRAINS OF TMEV. THE PRESENCE OF THR AT POSITION 202 HAS BEEN CONFIRMED BY THE SEQUENCING OF THE DA SEED STOCK (UNPUBLISHED DATA). ANOTHER DIFFERENCE BETWEEN THE SEQUENCE DATA BASE AND THE STRUCTURE PRESENTED IN THIS ENTRY IS AT RESIDUE 266 OF VP2 WHERE THE RESIDUE IN THE ENTRY WAS REFINED AS GLY INSTEAD OF ALA. THIS RESIDUE, THE CARBOXYL TERMINUS OF VP2, IS PARTLY DISORDERED AND NO SIDE CHAIN DENSITY WAS VISIBLE IN THE MAP.

THEILER'S MURINE ENCEPHALOMYELITIS VIRUS (DA STRAIN)