Crystal structure of AMP+PPi bound DesD, the desferrioxamine synthetase from the Streptomyces griseoflavus ferrimycin biosynthetic pathway

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

1-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

An acyl-adenylate mimic reveals the structural basis for substrate recognition by the iterative siderophore synthetase DesD.

0.05M Na PO4 pH 7.0,
0.2M Ammonium sulfate,
16% PEG 4000 (under oil 100%)

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

National Science Foundation (NSF, United States)

chain 'A' and (resid 1 through 157)

chain 'A' and (resid 158 through 410 )

chain 'A' and (resid 411 through 590 )

chain 'B' and (resid 1 through 157 )

chain 'B' and (resid 158 through 410 )

chain 'B' and (resid 411 through 592 )

chain 'C' and (resid 1 through 157 )

chain 'C' and (resid 158 through 410 )

chain 'C' and (resid 411 through 591)

chain 'D' and (resid 2 through 158 )

chain 'D' and (resid 159 through 410 )

chain 'D' and (resid 411 through 592 )

chain 'E' and (resid 2 through 157 )

chain 'E' and (resid 158 through 410 )

chain 'E' and (resid 411 through 591 )

NRPS-independent siderophore (NIS) synthetase, iterative synthetase, amide ligase, adenylate-forming enzyme, LIGASE