tRNA 2'-phosphotransferase (Tpt1) from Pyrococcus horikoshii in complex with NAD

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Structural basis for Tpt1-catalyzed 2'-PO 4 transfer from RNA and NADP(H) to NAD.

0.1 M Tris-Bicine (pH 8.5), 0.12 M divalent cations mixture (0.06 M each of magnesium chloride hexahydrate and calcium chloride dihydrate), 12.5 % MPD, 12.5% PEG-1000, and 12.5% PEG-3350

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

Department of Energy (DOE, United States)

lgand-free Tpt1 from P.horikoshii was used as a model

tRNA 2'-PHOSPHOTRANSFERASE, TPT1, tRNA SPLICING, NAD, TRANSFERASE