PAS-GAF bidomain of Glycine max phytochromeA

Structural insights into photoactivation and signalling in plant phytochromes.

0.05 M imidazole, 0.05 M MES, 0.02 M DL-Glutamatic acid monohydrate, 0.02 M DL-Alanine, 0.02M Glycine, 0.02 M DL-Lysine monohydrochloride, 0.02 M DL-Serine, 12% (v/v) Glycerol, 6% (w/v) PEG4000

This PAS-GAF bidomain of Glycine max phyA behaves as monomer in solution. The apparent molecular weight calculated from the elution peak from the gel filtraction chromatography corresponds to that of the monomer.

Hydrogens have been added in their riding positions