Crystal structure of alpha-galactosidase from Trichoderma reesei and its complex with galactose: implications for catalytic mechanism.

Crystallization of alpha-galactosidase from Trichoderma reesei.

PEG 6000, potassium phosphate, sodium phosphate , VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Data for Molecule - galactosidase, alpha-

SEQUENCE
Leu37, Pro70, Ala72, Ala88, Asn148, Phe151, Lys153, Thr161,
Asp165, Thr167, Gly185, His196, Met202, Gln207, Ser216,
Asp225, Asn230, Arg236, Leu238, Leu240, Leu245, Asp249,
Met258, Asn297, Asn300, Ile327, Val335, Tyr337, Phe341,
Val355, Ile360, Ala362, Thr363, Asn369, His378, Ser386,
Asp389, Ala398, Gln415, Arg416 differ from the sequence
database. These residues were used on the basis of the
electron density map. The difference may be due to
the use of different strains of Trichoderma reesei
for sequencing and for the X-ray structure.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

(beta/alpha)8 barrel, two domains, glycoprotein, HYDROLASE