Crystal structure of glutamine-dependent NAD+ synthetase from M. tuberculosis bound to AMP/PPi, NAD+, and glutamate

Regulation of the intersubunit ammonia tunnel in Mycobacterium tuberculosis glutamine-dependent NAD+ synthetase.

1.2 M ammonium citrate tribasic dihydrate, 5 % glycerol , pH 8.0, EVAPORATION, temperature 288K

The wild type protein bound to DON and NaAD+

The C176A mutant protein bound to AMPCPP and NaAD+

The C176A mutant protein bound to ATP and NaAD+

The C176A mutant protein bound to AMP/PPi, and NaAD+

Glutamine-dependent NAD(+) synthetase (E.C.6.3.5.1)

Glutamine-amidotransferase, Glutaminase, Glutamine-dependent NAD+ synthetase, Ligase, Ammonia tunneling, ATP binding, NAD, Nucleotide binding

chain A and (resseq 1:402 or resseq 409:543 or resseq 557:679 )

chain B and (resseq 1:402 or resseq 409:543 or resseq 557:679 )

chain C and (resseq 1:402 or resseq 409:543 or resseq 557:679 )

chain D and (resseq 1:402 or resseq 409:543 or resseq 557:679 )