Crystal structure of mKate S158A mutant at pH 7.5

{(4Z)-4-(4-hydroxybenzylidene)-2-[3-(methylthio)propanimidoyl]-5-oxo-4,5-dihydro-1H-imidazol-1-yl}acetic acid

Molecular Mechanism of a Green-Shifted, pH-Dependent Red Fluorescent Protein mKate Variant.

0.1M Tris-HCl, 0.25M ammonium citrate dibasic, 22% PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K

THIS SEQUENCE WAS ENGINEERED USING THE PROTEIN SEQUENCE FP480 FROM ENTACMAEA QUADRICOLOR (UNP D0VX33) AS THE ORIGINAL TEMPLATE

Fluoresent protein, pH sensor, FLUORESCENT PROTEIN