Bst DNA polymerase I Large Fragment wildtype D598A with 3'-amino primer, dGTP, and calcium time-resolved 1h

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Trivalent rare earth metal cofactors confer rapid NP-DNA polymerase activity

2M ammonium sulphate, 0.1M MES pH 5.3-6.0, 20 mM CaCl2, 5% MPD

DNA Polymerase, NP-DNA, Origin of Life, Time-Resolved Crystallography, REPLICATION-DNA complex