Versatile selective evolutionary pressure using synthetic defect in universal metabolism.

NADP-dependent isopropanol dehydrogenase

900 mM sodium citrate, 100 mM imidazole pH 8

Biotechnology and Biological Sciences Research Council

chain 'A' and (resid 1 through 150 )

chain 'A' and (resid 151 through 310 )

chain 'A' and (resid 311 through 352 )

chain 'B' and (resid 1 through 79 )

chain 'B' and (resid 80 through 150 )

chain 'B' and (resid 151 through 176 )

chain 'B' and (resid 177 through 210 )

chain 'B' and (resid 211 through 245 )

chain 'B' and (resid 246 through 310 )

chain 'B' and (resid 311 through 326 )

chain 'B' and (resid 327 through 352 )

chain 'C' and (resid 1 through 79 )

chain 'C' and (resid 80 through 210 )

chain 'C' and (resid 211 through 292 )

chain 'C' and (resid 293 through 355 )

chain 'D' and (resid 1 through 150 )

chain 'D' and (resid 151 through 292 )

chain 'D' and (resid 293 through 354 )