Non-square conformations of KtrA E125Q mutant rings with bound ADP

Activation of a nucleotide-dependent RCK domain requires binding of a cation cofactor to a conserved site.

100mM ADA pH 6.5, 4% PEG 4000, 20% glycerol

RCK domain, potassium homeostasis, cation channel, non-square conformation octameric ring, adp, TRANSPORT PROTEIN