Structure of s-Mgm1 decorating the outer surface of tubulated lipid membranes in the GTPgammaS bound state

Structure and assembly of the mitochondrial membrane remodelling GTPase Mgm1.

Half sets were generated not even-odd but as upper and lower-halves of particles in given tomogram

20 mM HEPES, 200 mM NaCl, residual MgCl2, 9mM KCl.

CTF determination was done by Gctf and correction was performed by ctfphaseflip from Imod

short isoform with C- and N-terminal truncations

Chaetomium thermophilum var. thermophilum DSM 1495

Sample was prepared in the described buffer. Just before freezing 6 nm colloidal gold fiducial marker were added to the sample in a 1:1 ratio.

Geometry-assisted particle picking form tube surfaces
global average of the particles rotated to the known initial orientations

Subtomogram average showing Mgm1 assembled on tubular membrane showing the whole tube

mitochondrial protein, membrane remodelling, GTPase, mitochondrial dynamics, membrane tubulation, contractile protein