Crystal structure of CobB Ac2 (A76G,I131C,V162A) in complex with H4K16Cr-2'OH-ADPr peptide intermediate after soaking

[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{S})-4-[(~{E})-but-2-enoxy]-3,5-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate

Evolved, Selective Erasers of Distinct Lysine Acylations.

0.1 mM Bis-Tris, 0.03M HCl, 23% PEG3350, soaked 36h with 1 mM NAD+

Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

NAD-dependent protein deacylase (E.C.3.5.1.-), Histone H4

deacylase, NAD-dependent, NAD, Intermediate, Crotonyl, Lysine, hydrolase

binding site for Di-peptide KMQ E 101 and LYS E 16

binding site for Di-peptide KMQ F 101 and LYS F 16

binding site for Di-peptide KMQ G 101 and LYS G 16

binding site for Di-peptide KMQ H 101 and LYS H 16