Crystal structure of a Fungal Catalase at 1.9 Angstrom

NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

Peroxisomal catalases from the yeasts Pichia pastoris and Kluyveromyces lactis as models for oxidative damage in higher eukaryotes.

SI(111) CHANNEL-CUT MONOCHROMATOR, CRYOCOOLED

The amino acid sequence of the N-terminal purification tag is: MKHHHHHHPMSDYDIPTTENLYFQGA.

N-terminal hexahistidine tag, cleavable with TEV protease

Very elongated, thin prisms and needles.

0.1 M sodium citrate, pH 5.5, 20% (w/v) polyethylene glycol (PEG) 3000

Spanish Ministry of Economy and Competitiveness

Glyceraldehyde-3-phosphate dehydrogenase (E.C.1.2.1.-)

Kluyveromyces lactis, Catalase, Heme, NADP, oxidoreductase