Cryo-EM structure of St1Cas9-sgRNA-tDNA20-AcrIIA6 dimeric assembly.

Cas9 Allosteric Inhibition by the Anti-CRISPR Protein AcrIIA6.

Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9)

in vitro transcription vector pT7-Fluc(deltai)

EMDB-4900 contains AcrIIA6 bound to St1Cas9-sgRNA-tDNA20 in monomeric form

Cryo-EM structure of St1Cas9-sgRNA-tDNA20-AcrIIA6 monomeric assembly.

EMDB-4902 contains St1Cas9-sgRNA-tDNA59-ntPAM proteins

EMDB-4904 contains AcrIIA6 bound to St1Cas9-sgRNA-tDNA59-ntPAM proteins

Atomic model built and refine on EMD-4900

Atomic model built and refine on EMD-4902

Atomic model built and refine on EMD-4904

AcrIIA6, CRISPR-associated endonuclease Cas9/RNA Complex
CRISPR-Cas9, anti-CRISPR protein, bacteriophages, Streptococcus thermophilus Cas9, St1Cas9, hydrolase