CryoEM structure of the plant helper NLR NRC2 in its resting state

Activation of plant immunity through conversion of a helper NLR homodimer into a resistosome

This is helper NLR protein NRC2 in inactive state with ADP nucleotide bound state

Tbhe autopick program picked so many particles including non-protein stuffs

The grids were negatively glow discharged

the particles are NRC2 purified from plant after over expression. This was suspended in buffer and cryogrids were made on graphene oxide backing

The C-flat grids were coated with grphene oxide, in house and the sample was applied two times inside the vitrobot chamber

This plasmid is compatible with agrobacterium tumifacians carrying NRC2 gene with 3xflag tag

Biotechnology and Biological Sciences Research Council (BBSRC)

CryoEM structure of the inactive plant helper NLR NRC2 from Nicotiana benthamiana

NLR protein, Helper NLRl, plant immunity, R-protein, IMMUNE SYSTEM